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Analysis of Whole Cell Lysate from the Intercellular Bacterium <i>Coxiella burnetii</i> Using Two Gel-Based Protein Separation Techniques

29 Jun 2007

Abstract excerpt

Coxiella burnetii, the causative agent of Q fever, is an obligate intracellular gamma-proteobacterium, which replicates within large phagolysosome-like compartments formed in the host cell. The global protein profile of intracellular C. burnetii strain Nine Mile phase II was analyzed by two gel-based approaches coupled to MALDI-TOF MS. Colloidal Coomassie brilliant blue-stained 2-DE gels at the pH range 3-10...

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PMID: 17602512
DOI: 10.1021/pr070077n

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