Article
Target genes and DNA-binding sites of the response regulator PhoR from Corynebacterium glutamicum.
Journal of bacteriology - 1 Jul 2007
Schaaf Sarah, Bott Michael
Abstract excerpt
The two-component signal transduction system PhoRS of Corynebacterium glutamicum is involved in the phosphate (P(i)) starvation response. To analyze the binding of unphosphorylated and phosphorylated PhoR to the promoters of phosphate starvation-inducible (psi) genes, this response regulator and the kinase domain of its cognate sensor, PhoS (MBP-PhoSDelta1-246), were overproduced and purified. MBP-PhoSDelta1-246...
Topics
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Corynebacterium glutamicum
- DNA Mutational Analysis
- DNA, Bacterial
- DNA-Binding Proteins
- Electrophoretic Mobility Shift Assay
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Mutation
- Phosphates
- Phosphorylation
- Promoter Regions, Genetic
- Protein Binding
- Sequence Analysis, DNA
- Sequence Homology, Nucleic Acid