Article
Expression of human glycophorin A in wild type and glycosylation-deficient Chinese hamster ovary cells. Role of N- and O-linked glycosylation in cell surface expression.
The Journal of biological chemistry - 15 Dec 1991
Remaley A T, Ugorski M, Wu N, Litzky L, Burger S R, Moore J S, Fukuda M, Spitalnik S L
Abstract excerpt
Glycophorin A, the most abundant sialoglycoprotein on human red blood cells, carries several medically important blood group antigens. To study the role of glycosylation in surface expression and antigenicity of this highly glycosylated protein (1 N-linked and 15 O-linked oligosaccharides), glycophorin A cDNA (M-allele) was expressed in Chinese hamster ovary (CHO) cells. Both wild type CHO cells and mutant CHO...
Topics
- Animals
- Antibodies, Monoclonal
- Blotting, Western
- CHO Cells
- Cell Membrane
- Cricetinae
- DNA
- Fluorescent Antibody Technique
- Glycophorins
- Glycosylation