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Article

[Cloning and inactivation of a chromosomal copy of the imidazole glycerophosphate dehydratase (HIS) gene from Hansenula polymorpha].

Molekuliarnaia genetika, mikrobiologiia i virusologiia - 1 Jul 1991

Semenova V D, Mikhaĭlover V M, Zlochevskiĭ M L, Lakhchev K, Beburov M Iu

Abstract excerpt

A gene for imidazole glycerophosphate dehydratase (HIS) has been selected from the library of Hansenula polymorpha genes by complementation of Escherichia coli hisB mutations or his3 mutation of Saccharomyces cerevisiae. Inactivation of the gene in Hansenula polymorpha strain 48V, as well as Leu(-)-Leu+ conversion of phenotype and arising of antibiotic G418 resistance resulted from transformation of the strain by...

Topics

Autoradiography
Blotting, Southern
Chromosomes, Fungal
Cloning, Molecular
DNA, Fungal
Electrophoresis, Agar Gel
Hydro-Lyases
Hydrogen-Ion Concentration
Hydrolysis
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleic Acid Hybridization

Pichia

Plasmids

Transformation, Genetic

Source

Identifiers

PMID: 1745264

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1745264-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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