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Article

Directed evolution of trimethoprim resistance in Escherichia coli

19 Apr 2007

Abstract excerpt

Directed evolution is a useful tool in the study of enzymes. It is used in this study to investigate the means by which resistance to the antibiotic trimethoprim develops in dihyrofolate reductase from Escherichia coli. Mutants with clinical levels of resistance were obtained after only three generations. After four generations of directed evolution, several mutants were characterized, along with some point...

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PMID: 17451440
DOI: 10.1111/j.1742-4658.2007.05801.x

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