Article
Postselective (directed) mutagenesis of fast-growing strain of Mycobacterium vaccae as a method of creating mutants showing changed phenotypic properties.
Acta microbiologica Polonica - 1 Jan 1994
Sajduda A, Dziadek J, Golańska E, Rumijowska A, Jaworski A
Abstract excerpt
Postselective (directed) mutagenesis was used to create mutants of M. vaccae B3805 showing changed abilities for steroid biotransformations. Three morphological classes of such mutants, differing in colony colour: yellow, white and pink, were selected. Some of them can be helpful in genetic studies of steroid biotransformation. Two mutants were also shown to be hosts for foreign DNA.
Topics
- Biotransformation
- DNA, Bacterial
- Kinetics
- Mutagenesis
- Mutation
- Mycobacterium
- Phenotype
- Plasmids
- Sitosterols
- Steroid Hydroxylases
- Steroids