Article
Reduction of protease inhibitor activity by expression of a mutant Bowman-Birk gene in soybean seed.
Plant molecular biology - 1 Jul 2007
Livingstone Donald, Beilinson Vadim, Kalyaeva Marina, Schmidt Monica A, Herman Eliot M, Nielsen Niels C
Abstract excerpt
A mutant Bowman-Birk gene was created that encoded an inactive high-sulfur product. It was used to transform soybean line Asgrow 3237. Transformants bearing the mutant gene were identified by GUS expression, PCR analysis, and Southern analysis. The amount of steady state mRNA from the mutant gene in the transformed plants showed that the gene was highly expressed, but the amount of message from the unmodified...
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