Article
Nanodiscs unravel the interaction between the SecYEG channel and its cytosolic partner SecA.
The EMBO journal - 18 Apr 2007
Alami Meriem, Dalal Kush, Lelj-Garolla Barbara, Sligar Stephen G, Duong Franck
Abstract excerpt
The translocon is a membrane-embedded protein assembly that catalyzes protein movement across membranes. The core translocon, the SecYEG complex, forms oligomers, but the protein-conducting channel is at the center of the monomer. Defining the properties of the SecYEG protomer is thus crucial to understand the underlying function of oligomerization. We report here the reconstitution of a single SecYEG complex...
Topics
- Adenosine Triphosphatases
- Bacterial Proteins
- Dimerization
- Electrophoresis, Polyacrylamide Gel
- Fluorescence Resonance Energy Transfer
- Lipid Bilayers
- Membrane Proteins
- Membrane Transport Proteins
- Mutation
- Nanostructures