Article
Evidence for delocalized anticooperative flash induced proton binding as revealed by mutants at the M266His iron ligand in bacterial reaction centers.
Biochemistry - 17 Apr 2007
Cheap Hélène, Tandori Julia, Derrien Valérie, Benoit Mireille, de Oliveira Pedro, Koepke Juergen, Lavergne Jérôme, Maroti Peter, Sebban Pierre
Abstract excerpt
Bacterial reaction centers (RCs) convert light energy into chemical free energy via the double reduction and protonation of the secondary quinone electron acceptor, QB, to the dihydroquinone QBH2. Two RC mutants (M266His --> Leu and M266His --> Ala) with a modified ligand of the non-heme iron have been studied by flash-induced absorbance change spectroscopy. No important changes were observed for the rate...
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