Article
Mutational control of bioenergetics of bacterial reaction center probed by delayed fluorescence.
Biochimica et biophysica acta - 1 Oct 2013
Onidas Delphine, Sipka Gábor, Asztalos Emese, Maróti Péter
Abstract excerpt
The free energy gap between the metastable charge separated state P(+)QA(-) and the excited bacteriochlorophyll dimer P* was measured by delayed fluorescence of the dimer in mutant reaction center proteins of the photosynthetic bacterium Rhodobacter sphaeroides. The mutations were engineered both at the donor (L131L, M160L, M197F and M202H) and acceptor (M265I and M234E) sides. While the donor side mutations...
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