Article
Mutations in the environment of the primary quinone facilitate proton delivery to the secondary quinone in bacterial photosynthetic reaction centers.
Biochemistry - 5 Jan 1999
Valerio-Lepiniec M, Miksovska J, Schiffer M, Hanson D K, Sebban P
Abstract excerpt
In Rhodobacter capsulatus, we constructed a quadruple mutant that reversed a structural asymmetry that contributes to the functional asymmetry of the two quinone sites. In the photosynthetically incompetent quadruple mutant RQ, two acidic residues near QB, L212Glu and L213Asp, have been mutated t...
Topics
- Alanine
- Benzoquinones
- Cytochrome c Group
- Electron Transport
- Glutamic Acid
- Kinetics
- Mutagenesis, Site-Directed
- Oxidation-Reduction
- Phenotype
- Photolysis
- Photosynthetic Reaction Center Complex Proteins
- Protons
- Rhodobacter capsulatus
- Tyrosine