Article
Differential proteolytic sensitivity of yeast fatty acid synthetase subunits alpha and beta contributing to a balanced ratio of both fatty acid synthetase components.
European journal of biochemistry - 1 Feb 1992
Schüller H J, Förtsch B, Rautenstrauss B, Wolf D H, Schweizer E
Abstract excerpt
The Saccharomyces cerevisiae genes FAS1 and FAS2 encoding the beta and alpha subunit of yeast fatty acid synthetase (FAS), respectively, were individually deleted by one-step gene disruption. Northern blot analysis of RNA from the resulting fas null allele mutants indicated that deletion of FAS2 did not influence the transcription of FAS1, while FAS2 transcription was significantly reduced in the delta fas1...
Topics
- Blotting, Northern
- Blotting, Western
- DNA, Recombinant
- Electrophoresis, Polyacrylamide Gel
- Fatty Acid Synthases
- Genes, Fungal
- Hydrolysis
- Mutation
- Protein Processing, Post-Translational
- RNA Processing, Post-Transcriptional