Article
Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR.
Journal of clinical microbiology - 1 May 2007
Bialasiewicz Seweryn, Whiley David M, Nissen Michael D, Sloots Theo P
Abstract excerpt
Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were f...
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