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Multiplexed Real-Time PCR Assay for Discrimination of <i>Plasmodium</i> Species with Improved Sensitivity for Mixed Infections

26 Feb 2009

Abstract excerpt

The implementation of real-time PCR for the diagnosis of malaria has been hampered by poor sensitivity for the detection of mixed infections. We have optimized a method that enhances the sensitivity of detection of minor species in mixed infections within a single multiplex reaction. Our assay uses species-specific forward primers in combination with a conserved reverse primer and largely overcomes primer...

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PMID: 19244467
DOI: 10.1128/jcm.01858-08

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