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Spurious Amplification of a <i>Plasmodium vivax</i> Small-Subunit RNA Gene by Use of Primers Currently Used To Detect <i>P. knowlesi</i>

8 Oct 2009

Abstract excerpt

The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes.

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PMID: 19812279
DOI: 10.1128/jcm.00811-09

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