Determination of the hepatitis C virus subtype: comparison of sequencing and reverse hybridization assays.

doi:10.1515/CCLM.2007.043

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Article

Determination of the hepatitis C virus subtype: comparison of sequencing and reverse hybridization assays.

Clinical chemistry and laboratory medicine - 1 Jan 2007

Stelzl Evelyn, van der Meer Carola, Gouw Remko, Beld Marcel, Grahovac Maja, Marth Egon, Kessler Harald H

Abstract excerpt

BACKGROUND: Hepatitis C virus (HCV) genotyping and accurate subtyping is becoming increasingly relevant to epidemiological studies, clinical management, pathogenicity, and vaccine development. METHODS: The TRUGENE HCV 5'NC Genotyping Kit, the new VERSANT HCV Genotype 2.0 Assay (LiPA), and a new laboratory-developed HCV NS5b sequencing assay designed for automated sequencing of the HCV NS5b region were used....

Topics

Genotype
Hepacivirus
Nucleic Acid Hybridization
Reagent Kits, Diagnostic
Sequence Analysis, DNA
Viral Nonstructural Proteins

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Identifiers

PMID: 17311502
DOI: 10.1515/CCLM.2007.043

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