Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamine.

doi:10.1128/jb.176.22.7079-7084.1994

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Article

Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamine.

Journal of bacteriology - 1 Nov 1994

Alexander D C, Valvano M A

Abstract excerpt

We report that rfe mutants of wild-type strains of Escherichia coli O7, O18, O75, and O111 did not express O-specific polysaccharide unless the rfe mutation was complemented by a cloned rfe gene supplied in a plasmid. The O polysaccharides in these strains are known to have N-acetylglucosamine (GlcNAc) in their O repeats. In addition, in vitro transferase assays with bacterial membranes from either the O7...

Topics

Acetylglucosamine
Agglutination
Blotting, Western
Carbohydrate Sequence
Enterobacteriaceae
Escherichia coli
Escherichia coli Proteins
Genes, Bacterial
Lipopolysaccharides
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

O Antigens

Polysaccharides, Bacterial

Terpenes

Transferases (Other Substituted Phosphate Groups)

Uridine Diphosphate Galactose

Uridine Diphosphate N-Acetylglucosamine

Source

Open in PubMed Open full text

Identifiers

PMID: 7525537
DOI: 10.1128/jb.176.22.7079-7084.1994
PMCID: PMC197083

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7525537-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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