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Article

Molecular cloning and genetic analysis of the rfb region from Shigella flexneri type 6 in Escherichia coli K-12.

FEMS microbiology letters - 1 Oct 1991

Cheah K C, Beger D W, Manning P A

Abstract excerpt

The rfb gene cluster which determines the biosynthesis of the Shigella flexneri serotype 6 O-antigen specificity has been cloned in pHC79, generating plasmids pPM3115 and pPM3116. These plasmids mediate expression, in Escherichia coli K-12, of lipopolysaccharides (LPS) immunologically similar to the S. flexneri type 6 LPS as judged by SDS-PAGE and Western-immunoblot analysis using S. flexneri type 6 specific...

Topics

Blotting, Southern
Blotting, Western
Cloning, Molecular
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Genotype
Lipopolysaccharides
Multigene Family
Phenotype
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sequence Homology, Nucleic Acid

Shigella flexneri

Source

Identifiers

PMID: 1778433
DOI: 10.1016/0378-1097(91)90356-f

Genes

SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1778433-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Molecular cloning and genetic analysis of the rfb region from Shigella flexneri type 6 in Escherichia coli K-12.PMID 1778433Read ↗

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