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Article

Natural substrate assay for chitinases using high-performance liquid chromatography: a comparison with existing assays.

Analytical biochemistry - 1 Apr 2007

Krokeide Inger-Mari, Synstad Bjørnar, Gåseidnes Sigrid, Horn Svein J, Eijsink Vincent G H, Sørlie Morten

Abstract excerpt

The determination of kinetic parameters of chitinases using natural substrates is difficult due to low K(m) values, which require the use of low substrate concentrations that are hard to measure. Using the natural substrate (GlcNAc)(4), we have developed an assay for the determination of k(cat) and K(m)values of chitinases. Product concentrations as low as 0.5 microM were detected using normal-phase...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 17288981
DOI: 10.1016/j.ab.2006.12.044

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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