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Article

A novel reverse-line hybridization assay for identifying genotypes of CTX-M-type extended-spectrum beta-lactamases.

The Journal of antimicrobial chemotherapy - 1 Mar 2007

Ensor V M, Livermore D M, Hawkey P M

Abstract excerpt

OBJECTIVES: To develop a reverse-line hybridization assay to identify CTX-M genotypes, potentially useful for large-scale investigation of surveillance collections. METHODS: Isolates carrying previously characterized bla(CTX-M) genes were used to develop the method. In addition, 334 isolates from five separate surveys were used to validate the method. CTX-M group was known from an independent multiplex PCR for...

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PMID: 17255146
DOI: 10.1093/jac/dkl505

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