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Article

Development of "oligotyping" for characterization and molecular epidemiology of TEM beta-lactamases in members of the family Enterobacteriaceae.

Antimicrobial agents and chemotherapy - 1 Nov 1990

Mabilat C, Courvalin P

Abstract excerpt

Based on the DNA sequences of blaTEM-1 and blaTEM-2, which encode parental penicillinases TEM-1 and TEM-2, respectively, and blaTEM-3, blaTEM-4, blaTEM-5, blaTEM-6, and blaTEM-7, which encode extended-spectrum beta-lactamases, we designed heptadecanucleotides to discriminate point mutations in five loci. Determination of the hybridization profiles by colony hybridization with this selection of probes, termed...

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Identifiers

PMID: 2073111
DOI: 10.1128/AAC.34.11.2210
PMCID: PMC172024

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