Article
Development of "oligotyping" for characterization and molecular epidemiology of TEM beta-lactamases in members of the family Enterobacteriaceae.
Antimicrobial agents and chemotherapy - 1 Nov 1990
Mabilat C, Courvalin P
Abstract excerpt
Based on the DNA sequences of blaTEM-1 and blaTEM-2, which encode parental penicillinases TEM-1 and TEM-2, respectively, and blaTEM-3, blaTEM-4, blaTEM-5, blaTEM-6, and blaTEM-7, which encode extended-spectrum beta-lactamases, we designed heptadecanucleotides to discriminate point mutations in fi...
Topics
- Amino Acids
- Base Sequence
- Culture Media
- Electrophoresis, Agar Gel
- Enterobacteriaceae
- Gene Amplification
- Isoelectric Focusing
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oligonucleotides
- Plasmids
- beta-Lactamases