Article
Rapid typing of group A streptococci by the use of DNA amplification and non-radioactive allele-specific oligonucleotide probes.
FEMS microbiology letters - 1 Jun 1994
Kaufhold A, Podbielski A, Baumgarten G, Blokpoel M, Top J, Schouls L
Abstract excerpt
Because of the allelic variations within the M protein gene (emm gene) of group A streptococci, reliable typing of this important human pathogen can be accomplished by the use of emm gene-specific oligonucleotide probes. Two technical modifications (a reverse dot blot and a reverse line blot hybr...
Topics
- Alleles
- Antigens, Bacterial
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Bacterial Typing Techniques
- Base Sequence
- Carrier Proteins
- Molecular Sequence Data
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Streptococcus pyogenes
