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Article

Rapid typing of group A streptococci by the use of DNA amplification and non-radioactive allele-specific oligonucleotide probes.

FEMS microbiology letters - 1 Jun 1994

Kaufhold A, Podbielski A, Baumgarten G, Blokpoel M, Top J, Schouls L

Abstract excerpt

Because of the allelic variations within the M protein gene (emm gene) of group A streptococci, reliable typing of this important human pathogen can be accomplished by the use of emm gene-specific oligonucleotide probes. Two technical modifications (a reverse dot blot and a reverse line blot hybridization assay) of a novel approach for the type-specific identification of emm genes have been developed. Both...

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PMID: 8039659
DOI: 10.1111/j.1574-6968.1994.tb06861.x

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