Article
Conserved beta-tubulin binding domain for the microtubule-associated motors underlying sperm motility and fast axonal transport.
Cell motility and the cytoskeleton - 1 Jan 1991
Goldsmith M, Connolly J A, Kumar N, Wu J, Yarbrough L R, van der Kooy D
Abstract excerpt
An antiserum against tubulin, NS20, has been previously shown to inhibit anterograde and retrograde axonal transport by 50% in vivo and in vitro. We report here that Protein A purified NS20 antibodies also attenuate sperm motility by 50% in demembranated sea urchin sperm. This inhibition is absorbed out by preincubating the NS20 antibodies with a biochemically purified porcine microtubule preparation, with...
Topics
- Amino Acid Sequence
- Animals
- Antibodies
- Axonal Transport
- Binding Sites
- Epitopes
- Male
- Microtubules
- Molecular Sequence Data
- Phenotype
- Recombinant Proteins