Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherichia coli K-12.

doi:10.1016/0378-1097(91)90274-e

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Article

Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherichia coli K-12.

FEMS microbiology letters - 15 Aug 1991

Morona R, Brown M H, Yeadon J, Heuzenroeder M W, Manning P A

Abstract excerpt

The rfb genes of Vibrio cholerae O1 (Ogawa serotype) were subcloned into a derivative of pBR322. This plasmid was transformed into several Escherichia coli K-12 mutant strains which produce an incomplete lipopolysaccharide (LPS)-core-oligosaccharide region. The data indicate that the V. cholerae...

Topics

Antigens, Bacterial
Carbohydrate Sequence
Cloning, Molecular
Escherichia coli
Glucose
Hemagglutination
Lipopolysaccharides
Molecular Sequence Data
Mutation
O Antigens
Vibrio cholerae

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