Triple immunofluorescence confocal laser scanning microscopy: spatial correlation of novel cellular differentiation markers in human muscle biopsies.

Abstract excerpt

Two different methods for triple immunofluorescence imaging with a confocal laser scanning microscope (CLSM) are described. The methods enable spatial "mapping" of 3 different epitope distribution patterns simultaneously in one tissue section. The key to triple imaging includes: (a) specific immunolabeling with 3 different mouse monoclonal antibodies (mAbs), (b) localization of the antibody binding sites by 3...

Topics

• Adult
• Antibodies, Monoclonal
• Biopsy
• Epitopes
• Female
• Fluorescent Antibody Technique
• Fluorescent Dyes
• Humans
• Male
• Microscopy, Electron
• Middle Aged
• Muscles