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Article

Adapting rice anther culture to gene transformation and RNA interference.

Science in China. Series C, Life sciences - 1 Oct 2006

Chen Caiyan, Xiao Han, Zhang Wenli, Wang Aiju, Xia Zhihui, Li Xiaobing, Zhai Wenxue, Cheng Zhukuan, Zhu Lihuang

Abstract excerpt

Anther culture offers a rapid method of generating homozygous lines for breeding program and genetic analysis. To produce homozygous transgenic lines of rice (Oryza sativa L.) in one step, we developed an efficient protocol of anther-callus-based transformation mediated by Agrobacterium after optimizing several factors influencing efficient transformation, including callus induction and Agrobacterium density for...

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Identifiers

PMID: 17172048
DOI: 10.1007/s11427-006-2013-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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