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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice.

Journal of visualized experiments : JoVE - 6 Oct 2020

Xu Dawei, Mondol Palash Chandra, Uzair Muhammad, Tucker Matthew R, Zhang Dabing

Abstract excerpt

Male sterility is an important agronomic trait for hybrid seed production that is usually characterized by functional defects in male reproductive organs/gametes. Recent advances in CRISPR-Cas9 genome editing technology allow for high editing efficacy and timesaving knockout mutations of endogenous candidate genes at specific sites. Additionally, Agrobacterium-mediated genetic transformation of rice is also a key...

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PMID: 33104071
DOI: 10.3791/61665

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