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Article

The role of N286 and D320 in the reaction mechanism of human dihydrolipoamide dehydrogenase (E3) center domain.

Journal of biomedical science - 1 Mar 2007

Wang Yi-Chun, Wang Shih-Tsung, Li Chuan, Liu Wen-Hu, Chen Pei-Ru, Chen Ling-Yun, Liu Te-Chung

Abstract excerpt

According to the multiple alignment of various dihydrolipoamide dehydrogenases (E3s) sequences, three human mutant E3s of the conserved residues in the center domain, N286D, N286Q, and D320N were created, over-expressed and purified. We characterized these mutants to investigate the reaction mechanism of human dihydrolipoamide dehydrogenases. The specific activities of N286D, N286Q, and D320N are 30.84%, 24.57%...

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Identifiers

PMID: 17171578
DOI: 10.1007/s11373-006-9136-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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