Article
Generating novel allelic variation through Activator insertional mutagenesis in maize.
Genetics - 1 Mar 2007
Bai Ling, Singh Manjit, Pitt Lauren, Sweeney Meredith, Brutnell Thomas P
Abstract excerpt
The maize transposable element Activator (Ac) has been exploited as an insertional mutagen to disrupt, clone, and characterize genes in a number of plant species. To develop an Ac-based mutagenesis platform for maize, a large-scale mutagenesis was conducted targeting the pink scutellum1 locus. We selected 1092 Ac transposition events from a closely linked donor Ac, resulting in the recovery of 17 novel ps1...
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