Article
Reconstitutional mutagenesis of the maize P gene by short-range Ac transpositions.
Genetics - 1 Aug 1992
Moreno M A, Chen J, Greenblatt I, Dellaporta S L
Abstract excerpt
The tendency for Ac to transpose over short intervals has been utilized to develop insertional mutagenesis and fine structure genetic mapping strategies in maize. We recovered excisions of Ac from the P gene and insertions into nearby chromosomal sites. These closely linked Ac elements reinserted into the P gene, reconstituting over 250 unstable variegated alleles. Reconstituted alleles condition a variety of...
Topics
- Alleles
- Base Sequence
- DNA
- DNA Mutational Analysis
- DNA Transposable Elements
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- Plants
- Zea mays