[Amplification of Ds flanking sequences from rice genomic DNA of hybrids of Ac x Ds lines and the analysis of Ds insertions].

Abstract excerpt

Ac and Ds insertions among the genomic DNAs of hybrids of Ac x Ds lines were screened by PCR. The genomic DNAs, which were proved to harbour both Ac and Ds, were used as templates in TAIL-PCR to clone the Ds flanking sequences. The cloned specific fragments were sequenced, and the sequenced Ds flanking sequences were used as query sequences to perform on-line sequence comparing analysis against GenBank by...

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