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Purification and characterization of trehalose-6-phosphate synthase from Saccharomycopsis fibuligera A11.

Indian journal of biochemistry & biophysics - 1 Oct 2006

Liang Likun, Chi Zhenming, Gao Lingmei, Ma Liyan

Abstract excerpt

Mutant A11, a mutant of Saccharomycopsis fibuligera Sdu with low acid and neutral trehalase was found to accumulate over 18% (w/w) trehalose from starch in its cells. In this study, trehalose-6-phosphate synthase (Tps1) was purified to homogeneity from this mutant, with a 30-fold increase in the specific enzyme activity, as compared to the concentrated cell-free extract, from initial cells. The molecular mass of...

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PMID: 17133735

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