Article
Disruption of the acid protease gene in Saccharomycopsis fibuligera A11 enhances amylolytic activity and stability as well as trehalose accumulation.
Enzyme and microbial technology - 10 Jun 2011
Wang Dongsheng, Chi Zhe, Zhao Shoufeng, Chi Zhen-Ming
Abstract excerpt
The acid protease gene in Saccharomycopsis fibuligera A11 was disrupted by integrating the HPT (hygromycin B phosphotransferase) gene into ORF (Open Reading Frame) of the acid protease gene. The mutant 12 obtained could grow in the medium containing hygromycin. No clear zone formed by the mutant grown on the plate containing milk protein was observed whereas a big clear zone formed by the strain A11 was detected....
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