Article
Acid tolerance of Streptococcus macedonicus as assessed by flow cytometry and single-cell sorting.
Applied and environmental microbiology - 1 Jan 2007
Papadimitriou Konstantinos, Pratsinis Harris, Nebe-von-Caron Gerhard, Kletsas Dimitris, Tsakalidou Effie
Abstract excerpt
An in situ flow cytometric viability assay employing carboxyfluorescein diacetate and propidium iodide was used to identify Streptococcus macedonicus acid tolerance phenotypes. The logarithmic-phase acid tolerance response (L-ATR) was evident when cells were (i) left to autoacidify unbuffered medium, (ii) transiently exposed to nonlethal acidic pH, or (iii) systematically grown under suboptimal acidic conditions...
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