Article
Direct demonstration of an adaptive constraint.
Science (New York, N.Y.) - 20 Oct 2006
Miller Stephen P, Lunzer Mark, Dean Antony M
Abstract excerpt
The role of constraint in adaptive evolution is an open question. Directed evolution of an engineered beta-isopropylmalate dehydrogenase (IMDH), with coenzyme specificity switched from nicotinamide adenine dinucleotide (NAD) to nicotinamide adenine dinucleotide phosphate (NADP), always produces mutants with lower affinities for NADP. This result is the correlated response to selection for relief from inhibition...
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