Article
Sugarcane (Saccharum spp.).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Arencibia Ariel D, Carmona Elva R
Abstract excerpt
We describe the procedures for recovering transgenic sugarcane from co-cultivation of both calli and in vitro plants with Agrobacterium tumefaciens. The correct tissue culture strategies and the use of super-binary vector or super-virulent strain are crucial for the successful sugarcane transformation. Both plant regeneration via calli culture and micropropagation strategies can be optimized to a wide spectrum of...
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