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Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane.

Plant cell reports - 1 Oct 2013

Mayavan Subramanian, Subramanyam Kondeti, Arun Muthukrishnan, Rajesh Manoharan, Kapil Dev Gnanajothi, Sivanandhan Ganeshan, Jaganath Balusamy, Manickavasagam Markandan, Selvaraj Natesan, Ganapathi Andy

Abstract excerpt

KEY MESSAGE: An efficient, reproducible and genotype-independent in planta transformation has been standardized for sugarcane using seed as explant. Transgenic sugarcane production through Agrobacterium infection followed by in vitro regeneration is a time-consuming process and highly genotype dependent. To obtain more number of transformed sugarcane plants in a relatively short duration, sugarcane seeds were...

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PMID: 23749098
DOI: 10.1007/s00299-013-1467-5

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