Article
Mutation of essential catalytic residues in pig citrate synthase.
Biochemistry - 21 Aug 1990
Alter G M, Casazza J P, Zhi W, Nemeth P, Srere P A, Evans C T
Abstract excerpt
Two amino acid residues, His274 and Asp375, were replaced singly in the active site of pig citrate synthase (PCS) with Gly274, Arg274, Gly375, Asn375, Glu375, and Gln375. The nonmutant protein and the mutant proteins were expressed in and purified from Escherichia coli, and the effects of these amino acid substitutions on the overall reaction rate and conformation of the PCS protein were studied by initial...
Topics
- Animals
- Antibodies, Monoclonal
- Aspartic Acid
- Binding Sites
- Catalysis
- Citrate (si)-Synthase
- Epitopes
- Escherichia coli
- Histidine
- Hydrogen Bonding
- Kinetics
- Mutation