A mutant of Escherichia coli citrate synthase that affects the allosteric equilibrium.

doi:10.1139/o91-035

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Article

A mutant of Escherichia coli citrate synthase that affects the allosteric equilibrium.

Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Apr 1991

Anderson D H, Donald L J, Jacob M V, Duckworth H W

Abstract excerpt

We describe a mutant of Escherichia coli citrate synthase, CS R319L, in which the arginine residue at position 319 of the sequence has been replaced by leucine. In this mutant, saturation by the substrate acetyl-CoA is changed from sigmoid (Hill parameter = 1.75 +/- 0.2) to hyperbolic (Hill param...

Topics

Allosteric Regulation
Arginine
Citrate (si)-Synthase
Coenzyme A
Escherichia coli
Glutamates
Glutamic Acid
Kinetics
Leucine
Mutagenesis, Site-Directed
Mutation
NAD
Oxaloacetates
Protein Conformation
Spectrophotometry, Ultraviolet

Source

Open in PubMed

Identifiers

PMID: 1675860
DOI: 10.1139/o91-035

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1675860-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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