Article
A novel endonuclease IV post-PCR genotyping system.
Nucleic acids research - 1 Jan 2006
Kutyavin Igor V, Milesi Dave, Belousov Yevgeniy, Podyminogin Mikhail, Vorobiev Alexei, Gorn Vladimir, Lukhtanov Eugeny A, Vermeulen Nicolaas M J, Mahoney Walt
Abstract excerpt
Here we describe a novel endonuclease IV (Endo IV) based assay utilizing a substrate that mimics the abasic lesions that normally occur in double-stranded DNA. The three component substrate is characterized by single-stranded DNA target, an oligonucleotide probe, separated from a helper oligonucleotide by a one base gap. The oligonucleotide probe contains a non-fluorescent quencher at the 5' end and fluorophore...
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