Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Crystallization of mutant forms of glutaredoxin Grx1p from yeast.

Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Sept 2006

Håkansson Kjell O, Østergaard Henrik, Winther Jakob R

Abstract excerpt

Glutaredoxin Grx1p from yeast was crystallized both as an independent protein and in a protein fusion with His-tagged yellow fluorescent protein (rxYFP). A glutathionylated C30S mutant of the 12 kDa Grx1p was crystallized in two different forms in PEG 4000 at low pH. These orthorhombic and monoclinic forms diffract to 2.0 A (synchrotron radiation) and 2.7 A (rotating-anode generator), respectively. In contrast,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Crystallization
Share to Crystallography, X-Ray

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Glutaredoxins

Share to Luminescent Proteins

Share to Oxidoreductases

Share to Recombinant Fusion Proteins

Share to Saccharomyces cerevisiae

Share to Saccharomyces cerevisiae Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 16946480
DOI: 10.1107/S1744309106031216
PMCID: PMC2242864

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Crystallization of mutant forms of glutaredoxin Grx1p from yeast.PMID 16946480Read ↗

Structure of glutaredoxin Grx1p C30S mutant from yeast.Semantic proximity Read ↗

Biochemical characterization of yeast mitochondrial Grx5 monothiol glutaredoxin.Semantic proximity Read ↗

Purification, crystallization and preliminary X-ray diffraction analysis of glutathionylated Trx1 C33S mutant from yeast.Semantic proximity Read ↗

Quantitative assessment of the determinant structural differences between redox-active and inactive glutaredoxins.Semantic proximity Read ↗

Differential expression and role of two dithiol glutaredoxins Grx1 and Grx2 in Schizosaccharomyces pombe.Semantic proximity Read ↗

A single glutaredoxin or thioredoxin gene is essential for viability in the yeast Saccharomyces cerevisiae.Semantic proximity Read ↗

Determinants of activity in glutaredoxins: an in vitro evolved Grx1-like variant of Escherichia coli Grx3.Semantic proximity Read ↗

A novel monothiol glutaredoxin (Grx4) from Escherichia coli can serve as a substrate for thioredoxin reductase.Semantic proximity Read ↗

N-terminus deletion affecting the preparation of soluble cyanobacterial glutaredoxin in Escherichia coli.Semantic proximity Read ↗

Cloning, overexpression, and characterization of glutaredoxin 2, an atypical glutaredoxin from Escherichia coli.Semantic proximity Read ↗

Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe-2S] clusters.Semantic proximity Read ↗

Crystallization and preliminary X-ray analysis of a truncated mutant of yeast nuclear thiol peroxidase, a novel atypical 2-Cys peroxiredoxin.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.