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Article

Purification, crystallization and preliminary X-ray diffraction analysis of glutathionylated Trx1 C33S mutant from yeast.

Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Jan 2009

Lou Xiaochu, Zhang Yaru, Bao Rui, Zhou Cong-Zhao, Chen Yuxing

Abstract excerpt

Thioredoxins (Trxs) are a family of small redox-active proteins that are found in all living organisms. In Saccharomyces cerevisiae, two cytosolic Trxs (Trx1 and Trx2) and one mitochondrial Trx (Trx3) have previously been identified. In this work, cytosolic Trx1 containing a C33S mutant was overexpressed, purified, glutathionylated and crystallized using the hanging-drop vapour-diffusion method. A set of X-ray...

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PMID: 19153453
DOI: 10.1107/S1744309108039316
PMCID: PMC2628853

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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