Article
Effects of increased voltage on resolution in preparative isoelectric focusing of myoglobin varia.
Electrophoresis - 1 Sept 2006
Bottenus Danny, Leatzow Dan, Ivory Cornelius
Abstract excerpt
IEF is a powerful technique which separates proteins and other amphoteric solutes in a pH gradient according to their pI's. The current work evaluates the effect on resolution of increasing electric fields in a novel preparative, vortex-stabilized electrophoresis device. In shallow gradients spanning one pH unit, the variants of myoglobin were separated at applied voltages from 10 to 15 kV. Digital imaging of...
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