Article
A method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation.
Stem cells (Dayton, Ohio) - 1 Dec 2006
Hasegawa Kouichi, Fujioka Tsuyoshi, Nakamura Yukio, Nakatsuji Norio, Suemori Hirofumi
Abstract excerpt
Human embryonic stem cells (hESCs) exhibit pluripotency and indefinite proliferation and are a potential source of cells for transplantation therapies and drug discovery. These applications will require large amounts of hESCs. However, hESCs are difficult to culture and maintain at larger scales, in part because of their low resistance to dissociation during passaging. To circumvent this, we developed a simple...
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