Article
Establishment and characterization of canine parvovirus-specific murine CD4+ T cell clones and their use for the delineation of T cell epitopes.
The Journal of general virology - 1 May 1990
Rimmelzwaan G F, van der Heijden R W, Tijhaar E, Poelen M C, Carlson J, Osterhaus A D, UytdeHaag F G
Abstract excerpt
Canine parvovirus (CPV)-specific T cell clones were generated by culturing lymph node cells from CPV-immunized BALB/c mice at limiting dilutions in the presence of CPV antigen and interleukin-2 (IL-2). All isolated T cell clones exhibited the cell surface phenotype Thy1+, CD4+, CD8- and prolifera...
Topics
- Amino Acid Sequence
- Animals
- Antigens, Viral
- CD4-Positive T-Lymphocytes
- Capsid
- Cells, Cultured
- Clone Cells
- Dogs
- Epitopes
- Feline Panleukopenia Virus
- Female
- Hypersensitivity, Delayed
- Interleukin-2
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Parvoviridae
- Phenotype