Article
Simplified polyacrylamide gel electrophoresis and silver staining for analysis and preparation of influenza virus RNA segments.
Journal of biochemical and biophysical methods - 1 Mar 1990
Abusugra I A, Linné T, Berg M
Abstract excerpt
The influenza virus has a genome consisting of eight RNA segments. A simplified technique to study the RNA segmental pattern by silver staining after gel electrophoresis has been developed. In addition, individual RNA segments could be isolated by a combination of polyacrylamide gel electrophoresis and isotachophoresis.
Topics
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Orthomyxoviridae
- RNA, Viral
- Silver
- Staining and Labeling
