Fine epitope mapping of the human SS-B/La protein. Identification of a distinct autoepitope homologous to a viral gag polyprotein.

doi:10.1172/JCI114606

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Article

Fine epitope mapping of the human SS-B/La protein. Identification of a distinct autoepitope homologous to a viral gag polyprotein.

The Journal of clinical investigation - 1 May 1990

Kohsaka H, Yamamoto K, Fujii H, Miura H, Miyasaka N, Nishioka K, Miyamoto T

Abstract excerpt

To analyze the autoepitopes on the SS-B/La protein, a cDNA covering the entire region coding the protein was isolated from a human cDNA library. The cDNA was subcloned into an expression plasmid vector, pEX, to express its protein product as a fusion protein with cro-beta-galactosidase in Escherichia coli. A recombinant pEX plasmid expressing three-fourths of the protein (amino acid 112-408) was also constructed....

Topics

Amino Acid Sequence
Autoantibodies
Autoantigens
Base Sequence
Chromatography, Affinity
Chromosome Deletion
Cloning, Molecular
DNA
Epitopes
Gene Library
Gene Products, gag

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Immunoblotting

Molecular Sequence Data

Mutation

Plasmids

Ribonucleoproteins

Sequence Homology, Nucleic Acid

Source

Open in PubMed Open full text

Identifiers

PMID: 1692037
DOI: 10.1172/JCI114606
PMCID: PMC296607

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1692037-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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