Article
Molecular profiling of the Clostridium leptum subgroup in human fecal microflora by PCR-denaturing gradient gel electrophoresis and clone library analysis.
Applied and environmental microbiology - 1 Aug 2006
Shen Jian, Zhang Baorang, Wei Guifang, Pang Xiaoyan, Wei Hua, Li Min, Zhang Yan, Jia Wei, Zhao Liping
Abstract excerpt
A group-specific PCR-based denaturing gradient gel electrophoresis (DGGE) method was developed and combined with group-specific clone library analysis to investigate the diversity of the Clostridium leptum subgroup in human feces. PCR products (length, 239 bp) were amplified using C. leptum cluster-specific primers and were well separated by DGGE. The DGGE patterns of fecal amplicons from 11 human individuals...
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