Article
Cell fusion of human and mouse cells as a source for new cells retaining human markers. Analysis of DNA content, membrane and cytoplasmic antigen expression.
Virchows Archiv. B, Cell pathology including molecular pathology - 1 Jan 1991
Lizard G, Chardonnet Y, Chignol M C, Thivolet J
Abstract excerpt
Flow cytometry and ultrastructural morphometry were used to study some characteristics of cells obtained by fusion with polyethylene-glycol 4000 between mouse fibroblasts 3T3.4E and normal keratinocytes (3T3.4E x NHK) or hand wart human keratinocytes (3T3.4E x HWK), at late passages. The cell cycle and the expression of human beta 2-microglobulin, human EGF-receptors (EGF-r), vimentin were simultaneously studied...
Topics
- 3T3 Cells
- Animals
- Antibodies, Monoclonal
- Antigens, Neoplasm
- Antigens, Surface
- Biomarkers
- Carcinoma, Squamous Cell
- Cell Cycle
- Cell Fusion
- Cell Nucleus
- Cells, Cultured