Article
Bacillus subtilis cytochrome oxidase mutants: biochemical analysis and genetic evidence for two aa3-type oxidases.
Molecular microbiology - 1 Aug 1991
van der Oost J, von Wachenfeld C, Hederstedt L, Saraste M
Abstract excerpt
The ctaBCDEF genes coding for cytochrome c oxidase were found to reside adjacent to a regulatory gene ctaA at 127 degrees on the Bacillus subtilis chromosome. The structural genes for subunits I and II, ctaD and ctaC, were deleted by gene-replacement using a phleomycin-resistance marker. The muta...
Topics
- Aspartic Acid
- Bacillus subtilis
- Blotting, Southern
- Chromosome Deletion
- Cytochrome c Group
- Cytochrome d Group
- Cytochromes
- Drug Resistance, Microbial
- Electron Transport Complex IV
- Escherichia coli
- Glucose
- Glutamates
- Glutamic Acid
- Mutation
- Phleomycins
- Plasmids
- Spectrophotometry
- Tetramethylphenylenediamine