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Article

Bacillus subtilis cytochrome oxidase mutants: biochemical analysis and genetic evidence for two aa3-type oxidases.

Molecular microbiology - 1 Aug 1991

van der Oost J, von Wachenfeld C, Hederstedt L, Saraste M

Abstract excerpt

The ctaBCDEF genes coding for cytochrome c oxidase were found to reside adjacent to a regulatory gene ctaA at 127 degrees on the Bacillus subtilis chromosome. The structural genes for subunits I and II, ctaD and ctaC, were deleted by gene-replacement using a phleomycin-resistance marker. The mutant was unable to oxidize N,N,N',N'-tetramethyl-p-phenylene-diamine and oxidized cytochrome c at a significantly lower...

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PMID: 1685007
DOI: 10.1111/j.1365-2958.1991.tb00829.x

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